Skip to main content
Hit enter to search or ESC to close
Close Search
Bio Molecular Systems
  • Products
    • Mic qPCR Cycler
    • Myra Liquid Handler
    • Mic IVD
  • About us
  • News
  • Login
  • Contact Us
  • Request a Demo

Kenya & USA: HRM analysis for identifying illegal-traded wildlife products.

  • February 17, 2021

Three-gene PCR and high-resolution melting analysis for differentiating vertebrate species mitochondrial DNA for biodiversity research and complementing forensic surveillance

Daniel O. Ouso, Moses Y. Otiende, Maamun M. Jeneby, Joseph W. Oundo, Joel L. Bargul, Scott E. Miller, Lillian Wambua & Jandouwe Villinger

International Centre of Insect Physiology and Ecology (icipe), P.O. Box 30772–00100, Nairobi, Kenya, Biochemistry Department, Jomo Kenyatta University of Agriculture and Technology (JKUAT), P.O. Box 62000–00200, Nairobi, Kenya, Kenya Wildlife Service, Veterinary Department, P.O. Box 40241-00100, Nairobi, Kenya, Institute of Primate Research, National Museums of Kenya, Department of Tropical and Infectious Diseases, P. O. Box 24481-00502, Karen, Nairobi, Kenya, National Museum of Natural History, Smithsonian Institution, Washington, DC, USA, International Livestock Research Institute, Department of Animal Biosciences, P.O. Box 30709-00100, Nairobi, Kenya|2020|Scientific Reports| 10:4741 https://doi.org/10.1038/s41598-020-61600-3

Illegal wildlife trade, including sale and consumption of bushmeat, leads to a significant decline of threatened wild animal species in Africa. Another consequence of illegal wildlife trade is the transmission of diseases that can cause serious health and economic concern. The ability to detect and prevent illegal wildlife trade requires accurate and efficient tools for species identification of confiscated and surveillance samples. The current testing that utilises DNA barcoding of cytochrome c oxidase I (COI) is costly when compared to standard PCR. To address this, a rapid, highly sensitive and cost-effective high-resolution melting (HRM) analysis-based approach was developed in this study. An HRM assay was developed on the Mic qPCR Cycler to differentiate 10 domestic and 24 wildlife species that are common in the East African illegal wildlife products trade from short mitochondrial COI, cytochrome b and 16S ribosomal RNA gene. All three HRM profiles of individual markers were combined to be able to characterise species. Mic’s first-class temperature uniformity can easily distinguish between small differences in Tm, as such in this study (<1°C). The assay was validated by blindly identifying illegal giraffe bushmeat among meat samples purchased from rural and urban butcheries. This was further confirmed by the gold standard forensic barcode sequencing. Therefore, the HRM assay presented in the study represents a valuable complement to molecular forensic pipelines for the surveillance of illegal wildlife products as it eliminates the need for mass barcode sequencing of suspect specimens.

Read More

  • Previous Project
  • Next Project

Ready to produce quality data faster?

DOWNLOAD THE SOFTWARE
REQUEST A DEMO
  • Mic
  • Myra
  • About us
  • News
  • Login/Register
Bio Molecular Systems

© 2023 Bio Molecular Systems – all rights reserved

  • BMS Privacy Policy
  • Mic qPCR Warranty
  • Myra Warranty
  • Contact
  • sales@biomolecularsystems.com

  • twitter
  • facebook
  • linkedin
  • youtube
Close Menu
  • Products
    • Mic qPCR Cycler
    • Myra Liquid Handler
    • Mic IVD
  • About us
  • News
  • Login
  • Contact Us
  • Request a Demo
Cookie NoticeThis website uses cookies to improve your user experience. Details on what cookies we use can be found in our privacy policy.
Accept
Read more
Manage Cookies
Cookie Box Settings
Cookie Box Settings
 

Privacy settings

Decide which cookies you want to allow.You can change these settings at any time. However, this can result in some functions no longer being available. For information on deleting the cookies, please consult your browser’s help function.Learn more about the cookies we use.

With the slider, you can enable or disable different types of cookies:

  • Block all
  • Essential
  • Functionality
  • Analytics
  • Advertising

This website will:

  • Essential: Remember your cookie permission setting
  • Essential: Allow session cookies
  • Essential: Gather information you input into a contact forms, newsletter and other forms across all pages
  • Essential: Authenticate that you are logged into your user account
  • Essential: Remember language version you selected

This website won't:

  • Remember your login details
  • Functionality: Remember social media settings
  • Functionality: Remember selected region and country
  • Analytics: Keep track of your visited pages and interaction taken
  • Analytics: Keep track about your location and region based on your IP number
  • Analytics: Keep track on the time spent on each page
  • Analytics: Increase the data quality of the statistics functions
  • Advertising: Tailor information and advertising to your interests based on e.g. the content you have visited before.
  • Advertising: Gather personally identifiable information such as name and location

This website will:

  • Essential: Remember your cookie permission setting
  • Essential: Allow session cookies
  • Essential: Gather information you input into a contact forms, newsletter and other forms across all pages
  • Essential: Authenticate that you are logged into your user account
  • Essential: Remember language version you selected
  • Functionality: Remember social media settings
  • Functionality: Remember selected region and country

This website won't:

  • Remember your login details
  • Analytics: Keep track of your visited pages and interaction taken
  • Analytics: Keep track about your location and region based on your IP number
  • Analytics: Keep track on the time spent on each page
  • Analytics: Increase the data quality of the statistics functions
  • Advertising: Tailor information and advertising to your interests based on e.g. the content you have visited before.
  • Advertising: Gather personally identifiable information such as name and location

This website will:

  • Essential: Remember your cookie permission setting
  • Essential: Allow session cookies
  • Essential: Gather information you input into a contact forms, newsletter and other forms across all pages
  • Essential: Authenticate that you are logged into your user account
  • Essential: Remember language version you selected
  • Functionality: Remember social media settingsl Functionality: Remember selected region and country
  • Analytics: Keep track of your visited pages and interaction taken
  • Analytics: Keep track about your location and region based on your IP number
  • Analytics: Keep track on the time spent on each page
  • Analytics: Increase the data quality of the statistics functions

This website won't:

  • Remember your login details
  • Advertising: Use information for tailored advertising with third parties
  • Advertising: Allow you to connect to social sites
  • Advertising: Identify device you are using
  • Advertising: Gather personally identifiable information such as name and location

This website will:

  • Essential: Remember your cookie permission setting
  • Essential: Allow session cookies
  • Essential: Gather information you input into a contact forms, newsletter and other forms across all pages
  • Essential: Authenticate that you are logged into your user account
  • Essential: Remember language version you selected
  • Functionality: Remember social media settingsl Functionality: Remember selected region and country
  • Analytics: Keep track of your visited pages and interaction taken
  • Analytics: Keep track about your location and region based on your IP number
  • Analytics: Keep track on the time spent on each page
  • Analytics: Increase the data quality of the statistics functions
  • Advertising: Use information for tailored advertising with third parties
  • Advertising: Allow you to connect to social sites
  • Advertising: Identify device you are using
  • Advertising: Gather personally identifiable information such as name and location

This website won't:

  • Remember your login details
Save & Close