Mic is Redefining
qPCR Performance
The Mic real-time qPCR cycler is engineered to deliver precision, speed, and reproducibility that redefine expectations in molecular testing. Its unique rotary design ensures exceptional thermal uniformity across all wells, eliminating edge effects and providing every sample with identical conditions. The result is sharper amplification plots, cleaner baselines, and unmatched consistency run after run.
Mic’s performance doesn’t stop at accuracy. With rapid heating and cooling rates, sensitive fluorescence detection, and seamless integration with intuitive software, Mic empowers researchers to generate high-quality data faster and with greater confidence. From gene expression studies to genotyping, HRM, and pathogen detection, Mic provides the reliability and performance you need in a compact, robust format that fits any lab.
Key Performance Metrics
Amazing results from a small package.
Melt curve analysis of the MnSOD gene amplification product (n=48)
Melt peak range = 84.44 – 84.52°C
Tm delta = 0.08°C
Uniformity measure of <±0.05°C
When it’s critical to distinguish small differences in Tm or Cq, temperature uniformity is at the top of your list. Mic’s superior temperature uniformity of ±0.05°C at zero seconds means we have you covered for all your melt analysis genotyping and real time quantification needs.
Class IV SNP (A to T)
Temperature difference between alleles < 0.10°C
Genotyping for single nucleotide polymorphisms (SNPs) or insertion-deletions (InDel), quantifying somatic mutations or epigenetic methylation levels, sequence matching for drug resistant bacteria, or gene scanning for new mutations, needs a tool like HRM. But not all HRM instruments are the same. Mic’s first class temperature uniformity means you can do these things with confidence no matter what HRM dye you choose (SYTO® 9, EvaGreen®, LC® Green).
Manganese superoxide dismutase gene (MnSOD)
Eight point, 2x dilution series of human genomic
Efficient = 98% (standard curve method)
R2 = 1.00
Whether it’s using standard curves for Absolute Quantification or determining gene expression using Relative Quantification through REST, know that the performance you get from the Mic will always ensure the highest level of quantitative accuracy.
10 point, 10x dilution series of Hepatitis B virus (HBV) cDNA template
Starting amount of 3E+09 copies (n = 3 each) over 10 logs
Efficiency = 95% (standard curve method)
R2 = 0.99
Have the power to detect high and low copy numbers of your target. Be it using Absolute Quantification to detect viral loads or simply determining a PCR efficiency using your standard curve, Mic will deliver the dynamic range you need, accurately and precisely.
Individual channel high power LEDs
Individual channel detectors
Cross talk <3% across all channels
If it’s molecular diagnostic (MDx) detection of pathogens, or genotyping using Allelic Discrimination, Mic has highly optimized filter sets to minimize your dye cross talk when multiplexing your real time PCR. With dedicated high powered LED and detectors per channel, detect all four colours in 2 sec during acquisition. No dye colour compensation or dye calibration needed – ever!
KRAS proto-oncogene, exon 2
Template amount was 200 copies/μL human genomic DNA (n = 48)
Two different instruments and three different experiments set up at
different times
CV across two instruments and runs = 6%
Mic’s reproducibility even extends to very low copy numbers. Critical in applications such as Relative Quantification, Absolute Quantification and Copy Number Variants (CNV).
Human Manganese Superoxide Dismutase (MnSOD) (n = 48)
Standard Deviation = 0.03
Cq Difference = 0.2
Efficieny = 98% (LinRegPCR method)
Be confident in the knowledge that each well is behaving identically to generate qPCR replicate data that is truly repeatable. Our focus on temperature uniformity means you can have confidence in your results whether its quantifying gene expression, determining genotypes, or measuring viral load.
Three different instruments and 3 different experiments set up at different times. CV across three instruments = 3%
We build our instruments to perfection so that each one is identical to the next. This means we can reproduce the same result not just across multi-runs, but instruments as well. Now you can combine multiple runs with minimal concern for variation. Inter-run calibrators become more of a quality control measure than a correction tool. And using sophisticated analysis software incorporating intelligent methods such as LinRegPCR, means we can further improve the qPCR reproducibility by minimising human error through analytical automation.
Five point dilution series of HBV plasmid cDNA template (n = 4 each)
5 picogram difference between standards (1.2 fold)
Efficiency = 98% (standard curve method)
R2 = 0.99
When you need to quantify small differences in relative gene expression, Mic will deliver the extreme levels of quantitative precision you need. Especially for bacterial genetics where minor differences in gene expression can multiply into big differences. This data shows a 5 picogram dilution series clearly differentiated with 0.2 cycles between standards!
Five point, 2x dilution series of Hepatitis B virus (HBV) cDNA template
Starting amount of 3E+06 copies (n = 4 each)
Efficiency = 90% (standard curve method); R2 = 0.99
Time to complete run (including melt) = 26 min
Get high quality data, fast! Mic’s speed is on par with the fastest instruments on the market. But unlike the competition Mic’s superior temperature uniformity and accuracy means you don’t sacrifice on the quality of your qPCR. Completing runs in under 30 minutes is the new standard with Mic, not the exception.
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Power your lab with automation that accelerates testing, reduces errors, and delivers consistent results.