Australia: Rapid and portable allele-specific qPCR for the analysis of fungicide resistance in wheat crops.
A quantitative allele-specific qPCR was developed on the portable Mic qPCR Cycler for the in-field quantification of the strobilurin‐resistant allele present in wheat crops.
Italy: Osteoarthritis-Infrapatellar Fat Pad exerts incomplete protective immunity from osteoarthritis inflammation
Structural and functional properties of stem cells isolated from osteoarthritic infrapatellar fat pad was charactered by TEM, flow cytometric analysis, cytochemistry and gene expression study performed on MIC qPCR Cycler
Germany & UK: Novel molecular approach for identification of toxicity in microbial community compositions during algal blooms in water reservoirs
qPCR and next generation using used to identify potential microcystin-producing species present during algal blooms in water reservoirs
USA, South Africa, Brazil & Uganda: Large-volume single-spin K2EDTA-plasma and EDTA-whole urine with up to 24 storage ideal for processing of pathogen cfDNA
Various preanalytical methods were investigated to optimize the processing of pathogen cfDNA in blood and urine samples.
Iran: Oleate deficiency affects fetal rat liver development
Gene expression of maturation markers in fetus liver were analysed using the Mic qPCR Cycler. The results show that combined MF-438 and oleate treatment increases gene expression, indicating that oleate availability during embryo development plays a part in fetal rat liver development.
New Zealand: DAZL-null male sheep lack germ cells whilst maintaining normal somatic cells for accelerated genetic improvements in livestock
Success of gRNA-Cas9 mediated homology-directed repair of DAZL loss-of-function mutation in male ovine fetal fibroblasts was confirmed via gene expression analysis performed on Mic qPCR Cycler.
Germany: Protein Kinase D required for combatting oxidative stress and development in Drosophila melanogaster
Relative quantification using the REST method provided by the micPCR software indicates that Protein Kinase D is required for development and survival in Drosophila melanogaster.
South Korea: A novel chlorhexidine-releasing polydimethylsiloxane coating for polymethyl methacrylate dental restorations was developed
A novel chlorhexidine-releasing polydimethylsiloxane coating for polymethyl methacrylate dental restorations was developed to inhibit bacterial activity.
Australia & UK: DDX5 promotes maintenance and function of spematogonia
Gene expression analysis on Mic qPCR Cycler and immunochemistry methods shows that DDX5 regulates appropriate splicing of key genes necessary for spermatogenesis and regulates expression of cell cycle genes in undifferentiated spermatogonia post-transcriptionally.
Germany & USA: PIAS1 is not suitable as a therapeutic agent for urothelial cancer
Gene expression analysis using Mic qPCR Cycler on PIAS1 demonstrates that it plays a minor role in cell survival and DNA repair within urothelial cancer cells in vitro and should not be used as a therapeutic agent.
USA: Sexual transmission of Zika virus typically only occurs in acutely infected mice.
Quantitative RT-qPCR was used to determine the possibility of sexual transmission of zika virus from infected males in chronic stages of disease. The expression levels of ZIKV RNA in intravaginal inoculated and subcutaneous inoculation groups of female and male mice were evaluated.
South Korea: Modular Chip for Bacterial DNA Extraction
A modular chip was developed to simplify and reduce the cost of bacterial DNA extraction. The chip demonstrated high efficiency and reproducibility with similar performance to conventional methods. It can potentially be used for Point-of-Care testing.
Italy: Infrapatellar Fat Pad stem cells exert incomplete protective activity from OA inflammation
The study employed techniques, such as gene expression performed on the MIC, to determine the structural and functional characterization of osteoarthritic infrapatellar fat pad stem cells and their role in osteoarthritic inflammation.
Poland: qPCR for the detection of Legionella spp. and E. Coli in hot tap water systems
In this study, L. spp., L. pneumophila, and E. coli bacteria were quantified from total DNA extracts of hot tap water samples using the MIC qPCR platform with TaqMan probes. The obtained results prove the presence of these bacteria in hot tap water installations in the majority of the examined buildings.
Finland: Molecular point-of-care testing for rapid diagnosis of common colds occurring in elite athletes
Molecular point-of-care testing (POCT) consists of a multiplex qPCR for rapid diagnosis of the aetiological agent causing the common cold. It allows for effective treatment and enhance timely isolation to decrease viral transmission.
Iran & USA: GO/Au nanocomposite enhances amplification in PCR systems
The effect of GO/Au nanocomposite was studied on end-point and real-time PCR (performed on the Mic Real-Time Cycler) employed for amplification of human GAPDH gene. The results showed that the GO/Au nanocomposite can improve both end-point and real time PCR methods at optimum concentrations, possibly through providing increased thermal convection by the GO surface as well as the Au nanostructures.
USA & China: Noninflammatory changes in Microglia contribute to epileptogenesis
The effects of electroconvulsive shock on microglia was measured by mRNA expression analysis, using two-step RT-qPCR performed on the Mic Real-Time Cycler. The results showed physiological changes in microglia but no neural injury, which may be relevant to electroconvulsive therapy.
Hungary: Severe kidney disease in Malaysian broiler chickens
A RT-qPCR, performed on the Mic Real-Time Cycler, was used to validate the identification of the etiological agent causing severe kidney disease in broiler chickens. The virus detected was found to be the virus Kedah fatal kidney syndrome virus (KFKSV).