Loop-mediated isothermal amplification was coupled with nanopore sequencing to provide rapid, high throughout diagnostics of SARS-CoV-2
A quantitative allele-specific qPCR was developed on the portable Mic qPCR Cycler for the in-field quantification of the strobilurin‐resistant allele present in wheat crops.
Structural and functional properties of stem cells isolated from osteoarthritic infrapatellar fat pad was charactered by TEM, flow cytometric analysis, cytochemistry and gene expression study performed on MIC qPCR Cycler
A rapid and mobile RT-qPCR diagnostic assay was developed for the differentiation of the four Dengue virus serotypes for timely intervention.
PCR amplification and RNA sequencing have suggested expression of Class I and Class II KNOX genes in the cambial zone of four Cactaceae species.
Transmission of ACFSVd in apples determined by ddPCR and standard curves generated by RT-qPCR for relative quantification.
Gene expression study of dopamine levels in CE-123 treated FASD rats shows CE-123 inhibits hyperactivity and improves cognitive abilities
Open-platform mobile laboratory for remote COVID-19 diagnosis using MYRA for sample processing and MIC for RT-qPCR
Gene expression analysis on the P125F substitution in NsLsi1 indicate that this unique reside reduces Si permeability in tobacco
Gene expression performed on the Mic qPCR Cycler shows down regulation of PARP1 transcription leads to repression of pluripotent transcription factors in human monocytes.
A rapid real-time PCR detection of R. solani AG-2-2, the causal agent of root rot in sugar beet. The Mic qPCR Cycler performs the same protocol as a conventional PCR in 1/4 of the time.
The light-weight and portable size of the Mic qPCR Cycler allows on-site real time PCR analysis of the mitochondrial DNA of six animal species in archaeological samples
Gene expression analysis using Mic qPCR Cycler on fumarase activity demonstrates that it plays a role in DNA damage response as well as metabolism and aerobic respiration of cells.
Gene expression analysis was performed on Mic qPCR Cycler to determine the cellular mediators of the cholesteatoma cells and its highly pro-inflammatory environment
qPCR and next generation using used to identify potential microcystin-producing species present during algal blooms in water reservoirs
Transcript profiling shows that RAM1 plays a role in late stages of transcriptional reprogramming of arbuscular mycorrhiza in Petunia hybrida
Combining HRM profiles of COI, Cytochrome b and 16S gene markers performed on the Mic qPCR Cycler allows discrimination between 10 domestic and 24 wildlife species.
A novel triplex qPCR assay for discrimination of Taenia species was developed and validated on the Mic qPCR Cycler
Various preanalytical methods were investigated to optimize the processing of pathogen cfDNA in blood and urine samples.
Gene expression of maturation markers in fetus liver were analysed using the Mic qPCR Cycler. The results show that combined MF-438 and oleate treatment increases gene expression, indicating that oleate availability during embryo development plays a part in fetal rat liver development.
Success of gRNA-Cas9 mediated homology-directed repair of DAZL loss-of-function mutation in male ovine fetal fibroblasts was confirmed via gene expression analysis performed on Mic qPCR Cycler.
Relative quantification using the REST method provided by the micPCR software indicates that Protein Kinase D is required for development and survival in Drosophila melanogaster.
A novel chlorhexidine-releasing polydimethylsiloxane coating for polymethyl methacrylate dental restorations was developed to inhibit bacterial activity.
Gene expression analysis on Mic qPCR Cycler and immunochemistry methods shows that DDX5 regulates appropriate splicing of key genes necessary for spermatogenesis and regulates expression of cell cycle genes in undifferentiated spermatogonia post-transcriptionally.
The efficiency of mRNA cellular uptake with PEF-OligoRNA/mRNAs was evaluated by relative quantification performed on the Mic qPCR Cycler
Quantitive RT-PCR was performed on the Mic Cycler to explore the role of the putzig gene in cells of germline origin.
The effects of myeloid-specific AMPK2 Subunit deletion were studied by gene expression and proteomic analysis performed on the Mic qPCR Cycler
A fast novel multiplex assay was developed on Mic qPCR Cycler for the detection of Leishmania spp.
Reduce qPCR run times to 30 minutes using Mic qPCR Cycler
Transcriptional, translation and protein analysis of Diamesa tonsa under warming conditions.
Novel SYBR Green qPCR assay for identification of Ca. P. solani and Ca. P. convolvuli in field bindweed
HRM analysis on the Mic qPCR cycler provides a quick and cost-effective test for detection of Q1a3a haplogroup in forensic samples.
Gene expression analysis using Mic qPCR Cycler on PIAS1 demonstrates that it plays a minor role in cell survival and DNA repair within urothelial cancer cells in vitro and should not be used as a therapeutic agent.
Quantitative RT-qPCR was used to determine the possibility of sexual transmission of zika virus from infected males in chronic stages of disease. The expression levels of ZIKV RNA in intravaginal inoculated and subcutaneous inoculation groups of female and male mice were evaluated.
In-field LAMP assay for detection of aprV2 Dichelobacter nodosus was compared to gold standard laboratory-based RT-qPCR assay performed on the Mic qPCR Cycler.
Gene expression analysis on three null alleles of cycG flies that were exposed to ionizing radiation treatment was performed on the Mic qPCR Cycler.
The role of the MAPK signaling pathway in U. compressa under chronic copper stress was investigated by studying the expression of selected genes using RT-qPCR performed on the MIC.
Role of DNA methylation in the TNF-α dependent regulation of ACE expression was studied using RT-qPCR on MIC qPCR cycler.
A modular chip was developed to simplify and reduce the cost of bacterial DNA extraction. The chip demonstrated high efficiency and reproducibility with similar performance to conventional methods. It can potentially be used for Point-of-Care testing.
The study employed techniques, such as gene expression performed on the MIC, to determine the structural and functional characterization of osteoarthritic infrapatellar fat pad stem cells and their role in osteoarthritic inflammation.
The effect of overexpression of Shh protein on hair follicle growth was investigated using quantitative qPCR on the Mic Real-Time Cycler.
The extent of function conservation of RecQ helicases was investigated using quantitative qPCR on the Mic Real-Time Cycler.
A propidium monoazide–qPCR (PMA-qPCR) method was validated using the Mic qPCR cycler for detecting viable Ascaris ova in wastewater
In this study, L. spp., L. pneumophila, and E. coli bacteria were quantified from total DNA extracts of hot tap water samples using the MIC qPCR platform with TaqMan probes. The obtained results prove the presence of these bacteria in hot tap water installations in the majority of the examined buildings.
Molecular point-of-care testing (POCT) consists of a multiplex qPCR for rapid diagnosis of the aetiological agent causing the common cold. It allows for effective treatment and enhance timely isolation to decrease viral transmission.
The effect of GO/Au nanocomposite was studied on end-point and real-time PCR (performed on the Mic Real-Time Cycler) employed for amplification of human GAPDH gene. The results showed that the GO/Au nanocomposite can improve both end-point and real time PCR methods at optimum concentrations, possibly through providing increased thermal convection by the GO surface as well as the Au nanostructures.
The effects of electroconvulsive shock on microglia was measured by mRNA expression analysis, using two-step RT-qPCR performed on the Mic Real-Time Cycler. The results showed physiological changes in microglia but no neural injury, which may be relevant to electroconvulsive therapy.
A RT-qPCR, performed on the Mic Real-Time Cycler, was used to validate the identification of the etiological agent causing severe kidney disease in broiler chickens. The virus detected was found to be the virus Kedah fatal kidney syndrome virus (KFKSV).