All Articles

Germany and Tunisia: Brucella in cerebrospinal fluid

A rapid real-time PCR assays using a Magnetic Induction Cycler (MIC) was established and validated for Brucella. Cerebrospinal fluid were obtained from patients diagnosed with encephalitis and meningitis in Tunisia hospitals between 2012-2017.

Australia: Multiplex qPCR for liver fluke (Fasciola hepatica) and intermediate snail host (Austropeplea tomentosa)

The design of a multiplex qPCR assay using MIC has allowed a highly sensitive and specific detection of Fasciola hepatica and its intermediate snail host, Austropeplea tomentosa, in water samples.

Korea: Protective effect of hydrolysates from seahorse on Atherosclerosis

After determining the most promising hydrolysate for an anti-atherosclerotic agent using seahorse, a qPCR using MIC was conducted to determine the effects of the hydrolysate on the expression of apoptosis-related genes.

Iraq: micro-RNA levels in follicular fluid indicate embryo quality

Real time PCR using MIC was used to determine if there is an association between miRNA-320 levels in follicular fluid and embryo quality in IVF-ISCI procedures. The miRNA levels in differing grades of embryo qualities was compared and an association was found with higher grade embryos containing more miRNA-320.

Australia, Thailand, Switzerland, Cambodia: Multiplex qPCR for Hookworms

The authors developed a multiplex qPCR for simultaneous detection of three Hookworm species. The ability to quantify results allows this newly developed assay to be used for monitoring and efficacy programs.

Germany and Tunisia: West Nile Virus in cerebrospinal fluid (CSF)

March 7, 2019

New Zealand: Selenium compounds as therapeutic agents in cancer patients

The study compared three oral selenium compounds to determine the dose and form that is the most effective and is safe to use as a therapeutic agent in cancer patients. A qPCR was used to determine the genotoxicity of the compounds.

Italy: Pemphigus Vulgaris

The authors established a High-resolution melting assay using the Magnetic Induction Cycler (MIC) for the identification of the ST18 rs17315309 gene functional risk variant for Pemphigus Vulgaris.

Japan – Eukaryotic Microorganism Genetics

Professor Kamakura Takashi uses the Mic for all of his groups work with eukaryotic microorganism genetics.

Uganda: Tapeworm (E. felidis)

November 13, 2018

Germany: Cyanobacteria

November 13, 2018

Germany: Notch Signalling Pathway

November 13, 2018

Israel: Synthetic Virology

The authors developed a methodology for analysing their synthetically derived viruses using the Mic.

Australia: Insertion Deletion Polymorphisms using HRM

The study showed that HRM on the Mic provided the best spatial separation of insertion deletion polymorphisms compared with the Rotor-Gene 6000 and CFX Connect.

Ireland: Meningitis/encephalitis

Multiplexed diagnostic qPCR assay for the detection of Neisseria meningitidis and other pathogens associated with meningitis/encephalitis.

Germany and UK: Middle ear disease

Real time PCR was used to measure gene expression levels of the pro-inflammatory genes TNFa and A20, along with the innate immune protein lipocalin 2 and the Toll like receptor 4, which is a mediator of inflammatory signalling

Australia: Livestock and Soils

Ian Marsh works at the Elizabeth Macarthur Agricultural Institute (EMAI, New South Wales Department of Primary Industries). This is the NSW Centre for Animal and Plant Biosecurity that undertakes plant and animal research and diagnostics.

New Zealand: Bee Virus

The design of new qPCR assays for trypanosomes and the use of existing primers together with our use of high resolution melting have confirmed the presence of both Lotmaria passim and several strains of the Lake Sinai Virus group in New Zealand.

Uganda: HIV Detection

ATCG uses the Magnetic Induction Cycler (MIC) real-time quantitative PCR instruments with a lower detection limit for circulating HIV-RNA of 1 copy per microliter of plasma.

Australia and Europe: Crop Disease Management

“From leaf samples using MIC, you can determine the genetic makeup of the pathogen population, determining not only the presence of genetic mutations that might affect fungicide performance but also the frequency of the population with that mutation”.

New Zealand: Honey Production

DNA is extracted from pollen present in honey and then tested using a multiplex qPCR assay (ManKan TM honey test) to detect the levels of Mānuka (Leptospermum scoparium) DNA. This method was developed and validated by dnature diagnostics & research Ltd and Scion Research as part of the Ministry for Primary Industries Mānuka Honey Science programme. The assay is one of the key tests that is used to verify authenticity of monofloral and multifloral Mānuka honey.

Vietnam: Taenia spp. Detection

This study describes the development and validation of a new triplex Taq-Man probe-based qPCR for the detection and discrimination of all three Taenia human tapeworms in human stools.