All Articles

Finland: Molecular point-of-care testing for rapid diagnosis of common colds occurring in elite athletes

Molecular point-of-care testing (POCT) consists of a multiplex qPCR for rapid diagnosis of the aetiological agent causing the common cold. It allows for effective treatment and enhance timely isolation to decrease viral transmission.

Iran & USA: GO/Au nanocomposite enhances amplification in PCR systems

The effect of GO/Au nanocomposite was studied on end-point and real-time PCR (performed on the Mic Real-Time Cycler) employed for amplification of human GAPDH gene. The results showed that the GO/Au nanocomposite can improve both end-point and real time PCR methods at optimum concentrations, possibly through providing increased thermal convection by the GO surface as well as the Au nanostructures.

USA & China: Noninflammatory changes in Microglia contribute to epileptogenesis

The effects of electroconvulsive shock on microglia was measured by mRNA expression analysis, using two-step RT-qPCR performed on the Mic Real-Time Cycler. The results showed physiological changes in microglia but no neural injury, which may be relevant to electroconvulsive therapy.

Hungary: Severe kidney disease in Malaysian broiler chickens

A RT-qPCR, performed on the Mic Real-Time Cycler, was used to validate the identification of the etiological agent causing severe kidney disease in broiler chickens. The virus detected was found to be the virus Kedah fatal kidney syndrome virus (KFKSV).

USA & China: Rapid diagnostic kit for accurate assessment of antifungal resistance of C. auris

A rapid diagnostic kit was developed for accurate and rapid assessment of FKS1-associated echinocandin resistance and ERG11-associated azole resistance in C. auris. The diagnostic platform used a duplex asymmetric PCR in conjunction with molecular beacon probe-based melting curve analysis that was performed on the Mic Real-Time PCR cycler.

Switzerland: ChIP assay for study of specific-nuclear signaling factors

In this study, a TF-ChIP protocol was developed to study specific nuclear signalling factors in cancers. The protocol produces high quality DNA output and can be reproduced using both fresh and fresh-frozen tissues, which is important in a clinical setting.

Canada & USA: Pathogenesis of Phytophthora sojae on soybean

Gene expression of the pathogenic genes in Phytophthora sojae were studied to further understand the gene-for-gene interaction between the pathogen and resistant soybean genes. In this study, a RT-qPCR was performed on the MIC and found that genomic signatures can predict phenotype compatibility between these genes.

Poland: Plant miRNAs in human breast milk

RT-qPCR experiments were performed on the MIC to identify the presence of five plant food-derived miRNAs that are present in human breast milk. Two of these miRNAs were found to be in exosomes and may influence crucial biological pathways in infants.

Switzerland: Metabolic regulation in Lactobacillus paracasei FAM18149

Next-generation sequencing and RT-qPCR performed on the MIC were utilized in this study to understand the metabolic pathway of sulfur-containing amino acids, cysteine and methionine, in Lactobacillus paracasei.

Switzerland: Effector molecules of pathogens prevent disease resistance in plants

In this study, the expression levels of the effecter molecule, RxLR24, present in Phytopthora pathogens were quantitated on the MIC. It was found that expression occurred early in the interaction between the pathogen and the plant and inhibits disease resistance in plants.

USA: Conserved epitopes between FIV and HIV

Identifying protective feline immunodeficiency virus (FIV) peptides that are evolutionarily conserved with human immunodeficiency virus (HIV) is useful in identifying protective HIV peptides that are required for successful HIV vaccines in humans.

Argentina: Drug resistance in breast cancer

In this study, a RT-qPCR on the MIC was used to analyse the expression of Acyl-CoA synthetase-4 (ACSL4), an enzyme involved in therapy resistance, and ATP-binding cassette (ABC) transporters, which detect and eject anti-cancer drugs from cells. It was concluded that ACSL4 can be used as a regulator of ABC transporters and increase the success of drug therapies in breast cancer.

Korea: Falcarindoil disrupts neural stem cell homeostasis

mRNA expression levels of neural stem cells (NSC) with FAD treatment was studied using RT-qPCR performed on the MIC. It was found that FAD suppresses the notch pathway of NSC, allowing excessive proliferation and differentiation.

USA & Brazil: Pyrethroid resistance in southern cattle fever ticks

An HRM assay, developed using the MIC, was successful in detecting mutations present in the para-sodium channel gene that is associated with pyrethroid resistance in southern cattle fever ticks.

New Zealand: Wobbly possum disease virus

The viral RNA loads of wobbly possum disease virus in experimentally infected possums were studied using RT-qPCR on the MIC. The study found high levels of RNA in lymph nodes, spleens and livers as well as moderate levels in serum and saliva of the infected possums.

USA: Sexual transmission of Zika virus

This study detected Zika virus in all components of the male reproductive tract by quantitative RT-PCR performed on the MIC. All components of the male reproductive tract were capable of sexually transmitting the Zika virus to naïve females.

Germany & UK: Cyclic di-AMP in Archaea

This study discovered the presence of the dacZ gene in an archaeal species, indicating production of c-di-AMP. The expression of dacZ was observed using qPCR, performed on the MIC, and found it constantly expressed at different growth phases. This provides evidence that the gene is essential in this archaeal species and thus, indicating c-di-AMP is also essential.

Iran and Serbia: Toxoplasma gondii

A quantitative PCR using the MIC Real-Time Cycler was used to validate an acute Toxoplasma gondii assay. 12.3% of 276 pregnant women in Salmas, North West of Iran, were identified as positive for acute T. gondii.

China, Australia and USA: Host-derived extracellular exosomes act as a defence mechanism during Haemonchus contortus parasitic infections.

RNA-sequencing of transcriptome responses in resistant and susceptible sheep flocks reveal 38 genes differentially expressed. The expression of these genes was further confirmed using qRT-PCR, performed on the MIC Real-Time Cycler.

USA, China & Canada: Zika virus polyclonal antibodies

Quantification of ZIKV infection, using the MIC Real-Time Cycler, on non-treated and treated hamsters with SAB-155 antibody revealed sufficient protection against the virus, even 4.5 days post infection.

Australia: Epidemiology of Toxoplasma gondii and the risk factor associated for Eastern Barred Bandicoot relocation on Phillip Island.

A qPCR, using MIC Real-Time Cycler, was used to study the prevalence of Toxoplasma gondii in feral cats and rabbits on Phillip Island to understand the associated risks for the introduction of the Eastern Barred Bandicoot.

Germany and Tunisia: Brucella in cerebrospinal fluid

A rapid real-time PCR assays using a Magnetic Induction Cycler (MIC) was established and validated for Brucella. Cerebrospinal fluid were obtained from patients diagnosed with encephalitis and meningitis in Tunisia hospitals between 2012-2017.

Australia: Multiplex qPCR for liver fluke (Fasciola hepatica) and intermediate snail host (Austropeplea tomentosa)

The design of a multiplex qPCR assay using MIC has allowed a highly sensitive and specific detection of Fasciola hepatica and its intermediate snail host, Austropeplea tomentosa, in water samples.

Korea: Protective effect of hydrolysates from seahorse on Atherosclerosis

After determining the most promising hydrolysate for an anti-atherosclerotic agent using seahorse, a qPCR using MIC was conducted to determine the effects of the hydrolysate on the expression of apoptosis-related genes.

Iraq: micro-RNA levels in follicular fluid indicate embryo quality

Real time PCR using MIC was used to determine if there is an association between miRNA-320 levels in follicular fluid and embryo quality in IVF-ISCI procedures. The miRNA levels in differing grades of embryo qualities was compared and an association was found with higher grade embryos containing more miRNA-320.

Australia, Thailand, Switzerland, Cambodia: Multiplex qPCR for Hookworms

The authors developed a multiplex qPCR for simultaneous detection of three Hookworm species. The ability to quantify results allows this newly developed assay to be used for monitoring and efficacy programs.

Germany and Tunisia: West Nile Virus in cerebrospinal fluid (CSF)

March 7, 2019

New Zealand: Selenium compounds as therapeutic agents in cancer patients

The study compared three oral selenium compounds to determine the dose and form that is the most effective and is safe to use as a therapeutic agent in cancer patients. A qPCR was used to determine the genotoxicity of the compounds.

Italy: Pemphigus Vulgaris

The authors established a High-resolution melting assay using the Magnetic Induction Cycler (MIC) for the identification of the ST18 rs17315309 gene functional risk variant for Pemphigus Vulgaris.

Japan – Eukaryotic Microorganism Genetics

Professor Kamakura Takashi uses the Mic for all of his groups work with eukaryotic microorganism genetics.

Uganda: Tapeworm (E. felidis)

November 13, 2018

Germany: Cyanobacteria

November 13, 2018

Germany: Notch Signalling Pathway

November 13, 2018

Israel: Synthetic Virology

The authors developed a methodology for analysing their synthetically derived viruses using the Mic.

Australia: Insertion Deletion Polymorphisms using HRM

The study showed that HRM on the Mic provided the best spatial separation of insertion deletion polymorphisms compared with the Rotor-Gene 6000 and CFX Connect.

Ireland: Meningitis/encephalitis

Multiplexed diagnostic qPCR assay for the detection of Neisseria meningitidis and other pathogens associated with meningitis/encephalitis.

Germany and UK: Middle ear disease

Real time PCR was used to measure gene expression levels of the pro-inflammatory genes TNFa and A20, along with the innate immune protein lipocalin 2 and the Toll like receptor 4, which is a mediator of inflammatory signalling

Australia: Livestock and Soils

Ian Marsh works at the Elizabeth Macarthur Agricultural Institute (EMAI, New South Wales Department of Primary Industries). This is the NSW Centre for Animal and Plant Biosecurity that undertakes plant and animal research and diagnostics.

New Zealand: Bee Virus

The design of new qPCR assays for trypanosomes and the use of existing primers together with our use of high resolution melting have confirmed the presence of both Lotmaria passim and several strains of the Lake Sinai Virus group in New Zealand.

Uganda: HIV Detection

ATCG uses the Magnetic Induction Cycler (MIC) real-time quantitative PCR instruments with a lower detection limit for circulating HIV-RNA of 1 copy per microliter of plasma.

Australia and Europe: Crop Disease Management

“From leaf samples using MIC, you can determine the genetic makeup of the pathogen population, determining not only the presence of genetic mutations that might affect fungicide performance but also the frequency of the population with that mutation”.

New Zealand: Honey Production

DNA is extracted from pollen present in honey and then tested using a multiplex qPCR assay (ManKan TM honey test) to detect the levels of Mānuka (Leptospermum scoparium) DNA. This method was developed and validated by dnature diagnostics & research Ltd and Scion Research as part of the Ministry for Primary Industries Mānuka Honey Science programme. The assay is one of the key tests that is used to verify authenticity of monofloral and multifloral Mānuka honey.

Vietnam: Taenia spp. Detection

This study describes the development and validation of a new triplex Taq-Man probe-based qPCR for the detection and discrimination of all three Taenia human tapeworms in human stools.