All Articles

Canada & India: P125 residue reduces Si permeability in tobacco plants

Gene expression analysis on the P125F substitution in NsLsi1 indicate that this unique reside reduces Si permeability in tobacco

Poland: Down regulation of PARP1 transcription in monocytes is controlled by cell cycle

Gene expression performed on the Mic qPCR Cycler shows down regulation of PARP1 transcription leads to repression of pluripotent transcription factors in human monocytes.

Serbia: Real-time PCR detection of R. solani AG-2-2

A rapid real-time PCR detection of R. solani AG-2-2, the causal agent of root rot in sugar beet. The Mic qPCR Cycler performs the same protocol as a conventional PCR in 1/4 of the time.

France: On-site screening of archaeological samples for DNA analysis.

The light-weight and portable size of the Mic qPCR Cycler allows on-site real time PCR analysis of the mitochondrial DNA of six animal species in archaeological samples

Israel & Singapore: Dual-targeted fumarase evolved before its dual location in eukaryotes

Gene expression analysis using Mic qPCR Cycler on fumarase activity demonstrates that it plays a role in DNA damage response as well as metabolism and aerobic respiration of cells.

Germany: Stem cells in cholesteatoma are cellular mediators of its highly pro-inflammatory environment

Gene expression analysis was performed on Mic qPCR Cycler to determine the cellular mediators of the cholesteatoma cells and its highly pro-inflammatory environment

Germany & UK: Novel molecular approach for identification of toxicity in microbial community compositions during algal blooms in water reservoirs

qPCR and next generation using used to identify potential microcystin-producing species present during algal blooms in water reservoirs

Switzerland & France: Transcript profiling of arbuscular mycorrhiza in Petunia hybrida

Transcript profiling shows that RAM1 plays a role in late stages of transcriptional reprogramming of arbuscular mycorrhiza in Petunia hybrida

Kenya & USA: HRM analysis for identifying illegal-traded wildlife products.

Combining HRM profiles of COI, Cytochrome b and 16S gene markers performed on the Mic qPCR Cycler allows discrimination between 10 domestic and 24 wildlife species.

Australia, Belgium & Vietnam: Novel triplex qPCR assay for discrimination of Taenia species

A novel triplex qPCR assay for discrimination of Taenia species was developed and validated on the Mic qPCR Cycler

USA, South Africa, Brazil & Uganda: Large-volume single-spin K2EDTA-plasma and EDTA-whole urine with up to 24 storage ideal for processing of pathogen cfDNA

Various preanalytical methods were investigated to optimize the processing of pathogen cfDNA in blood and urine samples.

Iran: Oleate deficiency affects fetal rat liver development

Gene expression of maturation markers in fetus liver were analysed using the Mic qPCR Cycler. The results show that combined MF-438 and oleate treatment increases gene expression, indicating that oleate availability during embryo development plays a part in fetal rat liver development.

New Zealand: DAZL-null male sheep lack germ cells whilst maintaining normal somatic cells for accelerated genetic improvements in livestock

Success of gRNA-Cas9 mediated homology-directed repair of DAZL loss-of-function mutation in male ovine fetal fibroblasts was confirmed via gene expression analysis performed on Mic qPCR Cycler.

Germany: Protein Kinase D required for combatting oxidative stress and development in Drosophila melanogaster

Relative quantification using the REST method provided by the micPCR software indicates that Protein Kinase D is required for development and survival in Drosophila melanogaster.

South Korea: A novel chlorhexidine-releasing polydimethylsiloxane coating for polymethyl methacrylate dental restorations was developed

A novel chlorhexidine-releasing polydimethylsiloxane coating for polymethyl methacrylate dental restorations was developed to inhibit bacterial activity.

Australia & UK: DDX5 promotes maintenance and function of spematogonia

Gene expression analysis on Mic qPCR Cycler and immunochemistry methods shows that DDX5 regulates appropriate splicing of key genes necessary for spermatogenesis and regulates expression of cell cycle genes in undifferentiated spermatogonia post-transcriptionally.

Japan: PEG-OligoRNA/mRNA complex stabilises Lipid Nanoparticles for In Vivo delivery

The efficiency of mRNA cellular uptake with PEF-OligoRNA/mRNAs was evaluated by relative quantification performed on the Mic qPCR Cycler

Germany: Putzig gene plays a critical role in germline stem cell development and differentiation

Quantitive RT-PCR was performed on the Mic Cycler to explore the role of the putzig gene in cells of germline origin.

Germany & Sweden: AMPK-alpha 2 Subunit Alters Monocyte Protein Expression and Atherogenesis

The effects of myeloid-specific AMPK2 Subunit deletion were studied by gene expression and proteomic analysis performed on the Mic qPCR Cycler

Australia: Novel multiplex assay utilizing bisulphite conversion for detection of Leishmania spp.

A fast novel multiplex assay was developed on Mic qPCR Cycler for the detection of Leishmania spp.

England: Rapid qPCR run times using Mic qPCR cycler

Reduce qPCR run times to 30 minutes using Mic qPCR Cycler

Italy: Heat shock regulation in cold stenothermal species

Transcriptional, translation and protein analysis of Diamesa tonsa under warming conditions.

Serbia & Switzerland: qPCR assay targeting Ca. P. solani and Ca. P. convolvuli

Novel SYBR Green qPCR assay for identification of Ca. P. solani and Ca. P. convolvuli in field bindweed

Argentina: HRM analysis for discrimination of native-American Y-haplogroup in DNA

HRM analysis on the Mic qPCR cycler provides a quick and cost-effective test for detection of Q1a3a haplogroup in forensic samples.

Germany & USA: PIAS1 is not suitable as a therapeutic agent for urothelial cancer

Gene expression analysis using Mic qPCR Cycler on PIAS1 demonstrates that it plays a minor role in cell survival and DNA repair within urothelial cancer cells in vitro and should not be used as a therapeutic agent.

USA: Sexual transmission of Zika virus typically only occurs in acutely infected mice.

Quantitative RT-qPCR was used to determine the possibility of sexual transmission of zika virus from infected males in chronic stages of disease. The expression levels of ZIKV RNA in intravaginal inoculated and subcutaneous inoculation groups of female and male mice were evaluated.

Australia: LAMP assay for detection of clinically virulent aprV2 Dichelobacter nodosus in-field

In-field LAMP assay for detection of aprV2 Dichelobacter nodosus was compared to gold standard laboratory-based RT-qPCR assay performed on the Mic qPCR Cycler.

Germany: CycG and p53 are required for DNA damage repair in Drosophila melanogaster

Gene expression analysis on three null alleles of cycG flies that were exposed to ionizing radiation treatment was performed on the Mic qPCR Cycler.

Italy: Infrapatellar Fat Pad stem cells exert incomplete protective activity from OA inflammation

The study employed techniques such as gene expression, performed on the MIC, to determine the structural and functional characterisation of osteoarthritic infrapatellar fat pad stem cells and their role in osteoarthritic inflammation.

Spain and Chile: MAPKs are key signaling mechanisms for detoxification in Macroalgae

The role of the MAPK signaling pathway in U. compressa under chronic copper stress was investigated by studying the expression of selected genes using RT-qPCR performed on the MIC.

Germany: DNA methylation determines TNF- α dependent regulation of ACE expression in human endothelial cells

Role of DNA methylation in the TNF-α dependent regulation of ACE expression was studied using RT-qPCR on MIC qPCR cycler.

South Korea: Modular Chip for Bacterial DNA Extraction

A modular chip was developed to simplify and reduce the cost of bacterial DNA extraction. The chip demonstrated high efficiency and reproducibility with similar performance to conventional methods. It can potentially be used for Point-of-Care testing.

Italy: Infrapatellar Fat Pad stem cells exert incomplete protective activity from OA inflammation

The study employed techniques, such as gene expression performed on the MIC, to determine the structural and functional characterization of osteoarthritic infrapatellar fat pad stem cells and their role in osteoarthritic inflammation.

South Korea: Sonic Hedgehog protein promotes hair-related gene expression

The effect of overexpression of Shh protein on hair follicle growth was investigated using quantitative qPCR on the Mic Real-Time Cycler.

USA: Functional conservation of RecQ helicase BLM between humans and Drosophila melanogaster

The extent of function conservation of RecQ helicases was investigated using quantitative qPCR on the Mic Real-Time Cycler.

Australia: A propidium monoazide–qPCR (PMA-qPCR) method for detection of viable Ascaris ova in wastewater

A propidium monoazide–qPCR (PMA-qPCR) method was validated using the Mic qPCR cycler for detecting viable Ascaris ova in wastewater

Poland: qPCR for the detection of Legionella spp. and E. Coli in hot tap water systems

In this study, L. spp., L. pneumophila, and E. coli bacteria were quantified from total DNA extracts of hot tap water samples using the MIC qPCR platform with TaqMan probes. The obtained results prove the presence of these bacteria in hot tap water installations in the majority of the examined buildings.

Finland: Molecular point-of-care testing for rapid diagnosis of common colds occurring in elite athletes

Molecular point-of-care testing (POCT) consists of a multiplex qPCR for rapid diagnosis of the aetiological agent causing the common cold. It allows for effective treatment and enhance timely isolation to decrease viral transmission.

Iran & USA: GO/Au nanocomposite enhances amplification in PCR systems

The effect of GO/Au nanocomposite was studied on end-point and real-time PCR (performed on the Mic Real-Time Cycler) employed for amplification of human GAPDH gene. The results showed that the GO/Au nanocomposite can improve both end-point and real time PCR methods at optimum concentrations, possibly through providing increased thermal convection by the GO surface as well as the Au nanostructures.

USA & China: Noninflammatory changes in Microglia contribute to epileptogenesis

The effects of electroconvulsive shock on microglia was measured by mRNA expression analysis, using two-step RT-qPCR performed on the Mic Real-Time Cycler. The results showed physiological changes in microglia but no neural injury, which may be relevant to electroconvulsive therapy.

Hungary: Severe kidney disease in Malaysian broiler chickens

A RT-qPCR, performed on the Mic Real-Time Cycler, was used to validate the identification of the etiological agent causing severe kidney disease in broiler chickens. The virus detected was found to be the virus Kedah fatal kidney syndrome virus (KFKSV).

USA & China: Rapid diagnostic kit for accurate assessment of antifungal resistance of C. auris

A rapid diagnostic kit was developed for accurate and rapid assessment of FKS1-associated echinocandin resistance and ERG11-associated azole resistance in C. auris. The diagnostic platform used a duplex asymmetric PCR in conjunction with molecular beacon probe-based melting curve analysis that was performed on the Mic Real-Time PCR cycler.

Switzerland: ChIP assay for study of specific-nuclear signaling factors

In this study, a TF-ChIP protocol was developed to study specific nuclear signalling factors in cancers. The protocol produces high quality DNA output and can be reproduced using both fresh and fresh-frozen tissues, which is important in a clinical setting.

Canada & USA: Pathogenesis of Phytophthora sojae on soybean

Gene expression of the pathogenic genes in Phytophthora sojae were studied to further understand the gene-for-gene interaction between the pathogen and resistant soybean genes. In this study, a RT-qPCR was performed on the MIC and found that genomic signatures can predict phenotype compatibility between these genes.

Poland: Plant miRNAs in human breast milk

RT-qPCR experiments were performed on the MIC to identify the presence of five plant food-derived miRNAs that are present in human breast milk. Two of these miRNAs were found to be in exosomes and may influence crucial biological pathways in infants.

Switzerland: Metabolic regulation in Lactobacillus paracasei FAM18149

Next-generation sequencing and RT-qPCR performed on the MIC were utilized in this study to understand the metabolic pathway of sulfur-containing amino acids, cysteine and methionine, in Lactobacillus paracasei.

Switzerland: Effector molecules of pathogens prevent disease resistance in plants

In this study, the expression levels of the effecter molecule, RxLR24, present in Phytopthora pathogens were quantitated on the MIC. It was found that expression occurred early in the interaction between the pathogen and the plant and inhibits disease resistance in plants.

USA: Conserved epitopes between FIV and HIV

Identifying protective feline immunodeficiency virus (FIV) peptides that are evolutionarily conserved with human immunodeficiency virus (HIV) is useful in identifying protective HIV peptides that are required for successful HIV vaccines in humans.