Skip to main content
Hit enter to search or ESC to close
Close Search
Bio Molecular Systems
  • Products
    • Mic qPCR Cycler
    • Myra Liquid Handler
    • Mic IVD
  • About us
  • News
  • Login
  • Contact Us
  • Request a Demo

USA, South Africa, Brazil & Uganda: Large-volume single-spin K2EDTA-plasma and EDTA-whole urine with up to 24 storage ideal for processing of pathogen cfDNA

  • February 15, 2021

Investigation of Preanalytical Variables Impacting Pathogen Cell-Free DNA in Blood and Urine

Kanagavel Murugesan, Catherine A. Hogan, Zaida Palmer, Byron Reeve, Grant Theron, Alfred Andama, Akos Somoskovi, Amy Steadman, Damian Madan, Jason Andrews, Julio Croda, Malaya K. Sahoo, Adithya Cattamanchi, Benjamin A. Pinsky, Niaz Banaei

Department of Pathology, Stanford University School of Medicine, Stanford, California, USA, Clinical Microbiology Laboratory, Stanford Health Care, Stanford, California, USA, NRF/DST Centre of Excellence for Biomedical Tuberculosis Research, South African Medical Research Council Centre for Tuberculosis Research, Division of Molecular Biology and Human Genetics, Faculty of Medicine and Health Sciences, Stellenbosch University, Cape Town, South Africa, College of Health Sciences, Makerere University, Kampala, Uganda, Global Health Technologies, Global Good Fund, Intellectual Ventures Laboratory, Bellevue, Washington, USA, Intellectual Ventures Laboratory, Bellevue, Washington, USA, Division of Infectious Diseases and Geographic Medicine, Department of Medicine, Stanford University School of Medicine, Stanford, California, USA, Faculty of Health Sciences, Federal University of Grande Dourados, Dourados, Brazil, Oswaldo Cruz Foundation, Campo Grande, Brazil, Department of Medicine, University of California, San Francisco, San Francisco, California, USA|2019|Journal of Clinical Microbiology|57: e00782-19 https://doi.org/10.1128/JCM.00782-19.

The impact of sample processing of pathogen cell-free DNA (pcfDNA) in blood and urine is currently not well understood. In this study, the impact of (i) blood collection tube and urine preservative, (ii) processing delay, (iii) processing method, (iv) freezing and thawing and (v) sample volume of pcfDNA on sensitivity of the pathogen pcfDNA detection was investigated. Blood and urine samples from healthy donors were spiked with cfDNA with different pathogens representative of bacteria, fungi and DNA viruses. A real-time PCR was performed using TaqMan probes on the Mic qPCR Cycler and the effects of each preanalytical process was measured using the PCR cycle threshold (Ct) and were compared to observe which methods provide lowest Ct values. The results suggest that large volume single spin K2EDTA-plasma and EDTA-whole urine with up to 24-hour processing delay may optimize pcfDNA detection. Further validation of the suggested sample processing method is required using clinical samples.

Read More

  • Previous Project
  • Next Project

Ready to produce quality data faster?

DOWNLOAD THE SOFTWARE
REQUEST A DEMO
  • Mic
  • Myra
  • About us
  • News
  • Login/Register
Bio Molecular Systems

© 2023 Bio Molecular Systems – all rights reserved

  • BMS Privacy Policy
  • Mic qPCR Warranty
  • Myra Warranty
  • Contact
  • sales@biomolecularsystems.com

  • twitter
  • facebook
  • linkedin
  • youtube
Close Menu
  • Products
    • Mic qPCR Cycler
    • Myra Liquid Handler
    • Mic IVD
  • About us
  • News
  • Login
  • Contact Us
  • Request a Demo
Cookie NoticeThis website uses cookies to improve your user experience. Details on what cookies we use can be found in our privacy policy.
Accept
Read more
Manage Cookies
Cookie Box Settings
Cookie Box Settings
 

Privacy settings

Decide which cookies you want to allow.You can change these settings at any time. However, this can result in some functions no longer being available. For information on deleting the cookies, please consult your browser’s help function.Learn more about the cookies we use.

With the slider, you can enable or disable different types of cookies:

  • Block all
  • Essential
  • Functionality
  • Analytics
  • Advertising

This website will:

  • Essential: Remember your cookie permission setting
  • Essential: Allow session cookies
  • Essential: Gather information you input into a contact forms, newsletter and other forms across all pages
  • Essential: Authenticate that you are logged into your user account
  • Essential: Remember language version you selected

This website won't:

  • Remember your login details
  • Functionality: Remember social media settings
  • Functionality: Remember selected region and country
  • Analytics: Keep track of your visited pages and interaction taken
  • Analytics: Keep track about your location and region based on your IP number
  • Analytics: Keep track on the time spent on each page
  • Analytics: Increase the data quality of the statistics functions
  • Advertising: Tailor information and advertising to your interests based on e.g. the content you have visited before.
  • Advertising: Gather personally identifiable information such as name and location

This website will:

  • Essential: Remember your cookie permission setting
  • Essential: Allow session cookies
  • Essential: Gather information you input into a contact forms, newsletter and other forms across all pages
  • Essential: Authenticate that you are logged into your user account
  • Essential: Remember language version you selected
  • Functionality: Remember social media settings
  • Functionality: Remember selected region and country

This website won't:

  • Remember your login details
  • Analytics: Keep track of your visited pages and interaction taken
  • Analytics: Keep track about your location and region based on your IP number
  • Analytics: Keep track on the time spent on each page
  • Analytics: Increase the data quality of the statistics functions
  • Advertising: Tailor information and advertising to your interests based on e.g. the content you have visited before.
  • Advertising: Gather personally identifiable information such as name and location

This website will:

  • Essential: Remember your cookie permission setting
  • Essential: Allow session cookies
  • Essential: Gather information you input into a contact forms, newsletter and other forms across all pages
  • Essential: Authenticate that you are logged into your user account
  • Essential: Remember language version you selected
  • Functionality: Remember social media settingsl Functionality: Remember selected region and country
  • Analytics: Keep track of your visited pages and interaction taken
  • Analytics: Keep track about your location and region based on your IP number
  • Analytics: Keep track on the time spent on each page
  • Analytics: Increase the data quality of the statistics functions

This website won't:

  • Remember your login details
  • Advertising: Use information for tailored advertising with third parties
  • Advertising: Allow you to connect to social sites
  • Advertising: Identify device you are using
  • Advertising: Gather personally identifiable information such as name and location

This website will:

  • Essential: Remember your cookie permission setting
  • Essential: Allow session cookies
  • Essential: Gather information you input into a contact forms, newsletter and other forms across all pages
  • Essential: Authenticate that you are logged into your user account
  • Essential: Remember language version you selected
  • Functionality: Remember social media settingsl Functionality: Remember selected region and country
  • Analytics: Keep track of your visited pages and interaction taken
  • Analytics: Keep track about your location and region based on your IP number
  • Analytics: Keep track on the time spent on each page
  • Analytics: Increase the data quality of the statistics functions
  • Advertising: Use information for tailored advertising with third parties
  • Advertising: Allow you to connect to social sites
  • Advertising: Identify device you are using
  • Advertising: Gather personally identifiable information such as name and location

This website won't:

  • Remember your login details
Save & Close