Sample Normalisation and qPCR Setup

The Sample Normalisation and qPCR Setup workflow supports concentration-based normalisation of samples before qPCR assay preparation. Samples with different starting concentrations are automatically diluted to a user-defined target concentration, providing consistent template input for real-time PCR and other PCR-based molecular testing applications.

The Myra Liquid Handling System automates diluent dispensing, intermediate dilution preparation, sample normalisation and mixing, reducing hands-on time and improving reproducibility. As an Independent Prep Protocol, the script can be loaded into the Workbench qPCR Setup application to combine sample normalisation and qPCR reaction setup within a single run. Dilution volumes and intermediate dilution steps are calculated automatically according to each sample’s starting concentration, target concentration and volume required for the configured qPCR reactions.

This MyraScript is available upon request. If you are interested in using this workflow in your laboratory, please contact support@biomolecularsystems.com to request a copy of the script.

Please note that this script has not yet undergone our full review and documentation process. Additional review, optimisation or validation may be required to ensure suitability for your specific application and laboratory setup.