MIC Checkerboard Assay

A checkerboard assay is a laboratory technique used to determine the potency and interaction of two antimicrobial compounds. By testing both agents in a matrix of varying serial dilutions, creating a checkerboard pattern, it identifies how the compounds work together to inhibit bacterial growth compared with their individual Minimum Inhibitory Concentrations (MICs).

The MIC Checkerboard Assay script automates preparation of the checkerboard plate by generating serial dilution series for both antimicrobial compounds before dispensing them into a 96- or 384-Well Plate to create the required concentration matrix. Media is automatically added to each dilution tubes and assay wells, with optional inoculum addition to complete the assay setup.

By automating the pipetting intensive steps, the Myra minimises hands on time, reduces the risk of manual pipetting errors and provides a consistent and reproducible assay setup while maintaining compatibility with standard broth microdilution checkerboard workflows.