Devyser CFTR

The Devyser CFTR workflow supports next-generation sequencing analysis of the CFTR gene from human genomic DNA. The assay enables detection of single nucleotide variants (SNVs), indels and copy number variations (CNVs) across the CFTR gene, supporting cystic fibrosis and CFTR-related genetic testing workflows. The workflow is compatible with Illumina MiSeq, MiniSeq and iSeq 100 sequencing platforms.

The Myra Liquid Handling System automates supported library preparation steps across three dedicated MyraScripts covering PCR1 library generation, PCR2 library indexing and library pooling and purification. Supporting the processing of up to 8 samples, automated reagent preparation, sample dilution, indexing preparation and library clean-up reduce hands-on time and improve reproducibility throughout the workflow. PCR amplification, Illumina sequencing and downstream data analysis are performed separately in accordance with the Devyser CFTR workflow.

This MyraScript is available upon request. If you are interested in using this workflow in your laboratory, please contact support@biomolecularsystems.com to request a copy of the script.

Please note that this script has not yet undergone our full review and documentation process. Additional review, optimisation or validation may be required to ensure suitability for your specific application and laboratory setup.