ADP-Glo Compound Screening

The ADP-Glo Compound Screening workflow supports kinase activity measurement, compound screening and inhibitor profiling applications. Using the ADP-Glo assay format, the workflow enables preparation of kinase reactions with test compounds, positive controls, negative controls and activity controls for luminescence-based kinase screening studies.

The Myra Liquid Handling System automates assay preparation, including kinase solution dispensing, compound transfer, ATP/substrate addition, ADP-Glo reagent addition and Kinase Detection Reagent addition, reducing hands-on time and improving reproducibility. User-configurable assay parameters support flexible replicate numbers, control configurations and source plate layouts, while automated incubation timing helps standardise kinase assay workflows. Optional off-deck shaking steps can be incorporated where required. Luminescence measurement and downstream data analysis are performed separately in accordance with the ADP-Glo assay workflow.

This MyraScript is available upon request. If you are interested in using this workflow in your laboratory, please contact support@biomolecularsystems.com to request a copy of the script.

Please note that this script has not yet undergone our full review and documentation process. Additional review, optimisation or validation may be required to ensure suitability for your specific application and laboratory setup.