CleanNGS Double Size Selection

CleanNGS Reagent was developed for the purification of PCR products or fragmentated DNA >100 bp from contaminants (e.g excess primers, nucleotides, salts and enzymes) and can also be used for double size selection in Next Generation Sequencing (NGS) applications. It utilises bead technology to selectively bind nucleic acids whereby the ratio of the magnetic beads controls what is retained on the beads and what remains in the supernatant. This allows for specific cutoffs that can be modified to suit reaction clean-up or double-sided size selection. Size selections can be performed quickly and easily to produce high-quality DNA suitable for highly sensitive applications including NGS, Cloning and Fragment Analysis. As the workflow uses beads, it makes it automation friendly. By automating the cleanup process, it provides a standardized workflow for improved results as well as reduction in costly errors and hands-on time.

This protocol automates CleanNGS for to 24 samples on the Myra using the Myra 96 Well Magnetic Station.