Australia: Insertion Deletion Polymorphisms using HRM

Comparison of 3 Methodologies for Genotyping of Small Deletion and Insertion Polymorphisms

Su Kah Goh, Ashan Musafer, Tom Witkowski, Vijayaragavan Muralidharan, Christopher Christophi, Hongdo Do, and Alexander Dobrovic | 2016 | Clinical Chemistry 62:7

Translational Genomics and Epigenomics Laboratory, Olivia Newton-John Cancer Research Institute, Heidelberg, Victoria, Australia; Department of Surgery, University of
Melbourne, Austin Health, Heidelberg, Victoria, Australia; School of Cancer Medicine, La Trobe University, Victoria, Australia; Department of Pathology, University of Melbourne, Victoria, Australia.

The study compared three different methods of determining insertion deletion polymorphisms; HRM, ddPCR and microfluidic electrophoresis. HRM proved to be the most robust approach to detecting insertion deletion polymorphisms due to its closed tube approach and speed. The group also compared the Mic to two other real-time platforms; Rotor-Gene 6000 (Corbett Research) and CFX Connect (Bio-Rad). The results demonstrated that rotor-based systems resulted in better special differentiation of alleles with the Mic providing the greatest separation.