Preparing a high-quality Next Generation Sequencing (NGS) library is a complex process that requires a significant investment of time, money, and expertise. The whole workflow typically consists of multiple stages, each with hundreds of pipetting steps that require precision. As pipetting precision depends on the user, variability is introduced which can negatively affect the reproducibility. Myra can streamline this labour-intensive process with minimal user intervention and setup time. We have provided a protocol to minimize programming and get you up and running quickly.

This protocol semi-automates the NEBNext Ultra II FS DNA Library Prep Kit for Illumina for up to 24 libraries on the Myra where the 96 Well Magnetic Station is used for the clean-up steps and the heating steps are performed on an external thermal cycler. By automating the pipetting-intensive steps, human error can be eliminated, thereby providing high-quality reproducible results, and increasing overall productivity.