Workbench Software for Mic qPCR Cycler
Advanced automated statistical
analysis to make your life easier.
Next generation qPCR analysis software that is user-friendly and packed with intelligent features.
Why wait long periods for results if you don’t have to? Mic PCR software allows you to see your results with detailed statistical analysis as soon as your run has completed.
Designed to meet MIQE specifications, our Mic PCR software offers you the most up-to-date qPCR analysis.
Take advantage of Mic PCR machine’s modular functionality and amazing reproducibility by combining multiple runs from multiple instruments into one analysis. With the ability to combine up to 10 runs you can analyse up to 480 samples at one time.
Mic qPCR software utilises qPCR genotyping to compare DNA sequences in real time. Mic’s real time (RT) PCR software analysis provides a simpler system for genotyping, without sacrificing efficiency for reliability.
Access to Mic PCR software means there is now no need to wait for samples to be batched into one 96 or 384 well run. Quantitative real time PCR cyclers complete the runs at the time you need them done and see the results now, not later. Take advantage of our media features and analyse traffic accurately.
Mic’s RQ software uses up-to-date mathematical models and well-founded statistical analysis, allowing you to accurately compare gene expression levels for different targets across multiple groups.
All the necessary calculations and statistics are carried out within the qPCR software. Data is reported both numerically and graphically.
With Mic’s superior thermal cycler temperature verification system you can easily detect differences between samples as little as 0.2 fold.
RQ with Myra
Use the Myra Liquid Handling System to easily setup all your Gene Expression RT-qPCR experiments.
Setup your assays, prepare your samples and master mixes then run Myra. Once the reactions are setup at the click of a button run the RT-qPCR on the Mic qPCR cycler and have the results automatically analysed at the end. Need to run more samples? No problem, just use Projects!
Watch this video to see just how easy it is to setup and run Relative Quantification on Mic and Myra.
High Resolution Melting Analysis
Mic’s qPCR analysis software offers an optional HRM analysis application that characterises DNA samples according to their melt behaviour allowing you to clearly identify mutations. HRM is the perfect tool for applications including determining allele prevalence, screening for loss of heterozygosity, DNA fingerprinting, DNA methylation, species identification and calculating the ratio of somatic acquired mutations.
Even difficult Class IV SNPs are no problem for Mic PCR software – the example here clearly shows the A base allele (red), T base allele (blue) and the heterozygote (purple).
Using a standard curve, the Mic PCR software’s AQ analysis application allows you to determine the absolute amount of a genetic target.
This five point, two fold dilution series produced an efficiency of 98%. The percentage variation between the given and calculated concentrations was no greater than 5% allowing for accurate quantification of the unknown sample.
Mic PCR software delineates differential melt curves from various types of chemistries, including quenched FRET dual hybridization probes, beacon probes or Plexor. This information can be used to characterise a sample’s genotype by converting array data into visual and meaningful results. Melt peaks can be inverted to accommodate the different chemistry types.
Use Mic PCR software to determine genotypes using dual labelled hydrolysis probes. With each probe designed toward a genetic variant, classify each genotype by using real time amplification data. This feature is particularly useful for instances where only a handful of single nucleotide polymorphisms need to be screened in hundreds of samples.
Employ Use the Assay library design feature to set up your target alleles and allow the software to call the unknowns automatically at the touch of a button.
The Identifier analysis uses a logic engine to help automate the identification of a target. The logic engine is a set of rules that will enable the qPCR software to make the identification.
Controls are also utilised to make appropriate decisions regarding the result of each sample and the run as a whole. These rules can be pre-defined for each target used in the assay individually or as a whole group of targets.
Identifier is a great tool for anyone wanting to do their own in-house diagnostics.
Utilising qPCR analysis software shouldn’t be time-consuming or overtly complex. Discover the difference with our lightweight PCR machine and detection system today.