Germany and Tunisia: West Nile Virus in cerebrospinal fluid (CSF)

Serological and Molecular Biological Detection of West Nile Virus in the Cerebrospinal Fluid of Patients of the Military Hospital of Tunisia, 2012-2017

R Ben Helal, S Mzoughi, M Diehl, G Zikeli, S Handrick, H Naija, M El Asli, F Barguellil, R Woelfel and M Ben Moussa | Military Hospital of Tunisia, Microbiology, Tunis, Tunisia; Bundeswehr Institute of Microbiology, Microbiology, Munich, Germany | 2018 | Global Partners in Biosecurity

Two real-time PCRs detecting the presence of West Nile Virus (WNV) lineage 1 and 2 strains in cerebrospinal fluid was established and validated on the Magnetic Induction Cycler (MIC). The cerebrospinal fluid collected from patients hospitalized in diverse services of the military hospital of Tunisia. The study indicates the establishment of rapid and sensitive RT-PCR assays for the detection of WNV lineages.