Overview
Preparing a high-quality Next Generation Sequencing (NGS) library is a complex process that requires a significant investment of time, money, and expertise. The whole workflow typically consists of multiple stages, each with hundreds of pipetting steps that require precision. As pipetting precision depends on the user, variability is introduced which can negatively affect the reproducibility. Myra can streamline this labour-intensive process with minimal user intervention and setup time. We have provided a protocol to minimize programming and get you up and running quickly.
This protocol semi-automates the Illumina DNA Prep for up to 24 libraries on the Myra where the 96 Well Magnetic Station is used for the clean-up steps and the heating steps are performed on an external thermal cycler. By automating the pipetting-intensive steps, human error can be eliminated, thereby providing high-quality reproducible results, and increasing overall productivity.
More information on the protocol can be found via Download the Protocol PDF.
Revision History
| Date | Revision |
|---|---|
| 25 July 2023 | Initial version |
| 1 November 2023 | Up to 24 samples can be processed. Can now select PCR Amplicon as DNA input for Illumina DNA Prep – Stage 2 – Clean Up. Option to determine where samples are on sample input plate in Illumina DNA Prep – Stage 1 – Tagmentation. Improved mixing to increase final yield produced. |
| 6 February 2024 | Split Stage 2 into two different templates depending on DNA input. |
| 9 December 2024 | Resolved an issue where the same tip was incorrectly reused for piercing the foil of subsequent samples. |