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AMPure XP PCR Cleanup

$date_functionality = (isset($nectar_options['post_date_functionality']) && !empty($nectar_options['post_date_functionality'])) ? $nectar_options['post_date_functionality'] : 'published_date'; if( '1' !== $remove_single_post_date ) { if( 'last_editied_date' === $date_functionality ) { echo ''.get_the_modified_date().''; } else { $nectar_u_time = get_the_time('U'); $nectar_u_modified_time = get_the_modified_time('U'); if( $nectar_u_modified_time >= $nectar_u_time + 86400 ) { echo '' . get_the_date() . ''; echo '' . get_the_modified_time(__( 'F jS, Y' , 'salient' )) . ''; } else { echo '' . get_the_date() . ''; } } } if($post_header_style != 'default_minimal') { echo ''.apply_filters('nectar_blog_page_header_categories',nectar_get_category_list(',')) .''; } else { echo ''. get_comments_number_text( esc_html__('No Comments', 'salient'), esc_html__('One Comment', 'salient'), esc_html__('% Comments', 'salient') ) . ''; } $remove_reading_dur = ( isset( $nectar_options['blog_remove_single_reading_dur'] ) ) ? $nectar_options['blog_remove_single_reading_dur'] : '0'; if( '1' !== $remove_reading_dur ) { echo ''.nectar_estimated_reading_time(get_the_content()). ' '. esc_html__('min read','salient').''; } ?>

| --> August 18, 2023 | Myra Scripts, Nucleic Acid Extraction

Overview

AMPure XP Reagent was developed for the purification of PCR products or fragmentated DNA >100 bp from contaminants (e.g excess primers, nucleotides, salts and enzymes) and is one of the most widely used workflows for DNA cleanup for different genomic applications like NGS, PCR, Cloning and Fragment Analysis. It utilizes Solid Phase Reversible Immobilisation (SPRI) paramagnetic bead technology to selectively bind nucleic acids by type and size, making it automation friendly. By automating the cleanup process, it provides a standardized workflow for improved results as well as reduction in costly errors and hands-on time.

This protocol automates AMPure XP Reagent for to 24 samples on the Myra using the Myra 96 Well Magnetic Station.

More information on the protocol can be found via Download the Protocol PDF.

Revision History

 

DateRevision
18 August 2023Initial version
26 October 2023

Improved mixing of sample and beads to increase final yield produced.

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