ADPGlo Assay

The ADP-Glo™ Kinase Assay is a luminescent assay that measures the amount of adenosine diphosphate (ADP) produced by kinase reactions over time. It is used to evaluate the activity of kinase enzymes and to screen for kinase inhibitors by quantifying the conversion of ATP to ADP in a time-course manner, making it a valuable tool for drug discovery and development programs targeting kinase-driven pathways.

This protocol enables execution of ADP-Glo™ kinase inhibitor dose response assays in a 96-well or 384-well format, with user-configurable options for inhibitor concentration ranges, dilution range and factor, replicate number, reaction volumes and incubation settings. The Myra automates pipetting-intensive steps, whilst luminescence detection is performed on a compatible plate reader. The outcome is a reproducible workflow suitable for compound screening, inhibitor profiling, and assay development.