Myra for Bead Cleanup
Bead clean up is a tedious and complex task required by every single lab doing NGS. For bead clean up Myra is as always easy to use, accurate, fast, efficient, and cost effective. Because pipetting precision is user-dependent, it can introduce variability that may affect reproducibility.
Thus, automation of bead clean up guarantees consistent and precise protocol execution, reducing the likelihood of user-errors. This enhanced reproducibility is crucial for maintaining data quality and consistency.
Principle
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DNA cleanup can be used for different genomic applications like
NGS, PCR, Cloning and Fragment Analysis. It utilizes Solid Phase Reversible Immobilisation (SPRI) paramagnetic bead technology to selectively bind nucleic acids by type and size, making it automation friendly. By automating the cleanup process, it provides a standardized workflow for improved results as well as reduction in costly errors and hands-on time.
Bead Cleanup on Myra
Browse a comprehensive table of all available templates on Myra, complete with direct links to specific MyraScript pages. Access protocol documentation via a dedicated PDF link. Verified options include templates for BMS, Partner (kit company), and the Myra community (customers).
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Case Study: CleanNGS Bead Clean Up
Efficiency of DNA purification using CleanNGS beads (CleanNA, Netherlands) was evaluated on the Myra liquid handling system in comparison to manual purification.
Experimental Setup
Bead Cleanup Protocol
1. Pipette 22 L of beads into a 200 L PCR plate
2. Thoroughly mix beads with sample to bind DNA
3. Capture beads using magnets (3 min)
4. Perform 2x wash steps with 100 uL of 80% Ethanol to remove impurities and contaminants
5. Air-dry to remove residual traces of Ethanol (5 min)
6. Pipette 21 L of elution buffer to the beads and release magnets
7. Resuspend beads and mix with the elution buffer to release DNA
8. Capture beads using magnets (3 min)
9. Elute 15 ML of purified DNA into new tubes
For the experiment, a PC product (550 bp) was purified in sets of 8 replicates, using the two different methods in parallel (Myra and manual). For the manual purification, the exact same protocol was performed by hand. Purification yield and purity were compared using the Qsep100 Capillary Electrophoresis System and S2 Quantitative Cartridge (BiOptic Inc., Taiwan). All experiments were conducted by our Swiss partner Labgene Scientific.Inc., Taiwan). All experiments were conducted by our Swiss partner Labgene Scientific.
Results
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The data clearly showed the Myra achieved significantly higher yields of purified PC product compared with the manual method (Table 1).
Reproducibility of yield was also tighter when using the Myra.
Fragment analyzer results showed correct fragment sizes across all 16 PCR samples purified using both the Myra and manual methods (Figure 1).
The results highlight the advantages of using Myra for bead clean up, as it not only increases the DNA vield but also makes the results more accurate and consistent. Myra automation reduces operator stress levels and allows you to focus on your research.