Downregulation of PARP1 transcription by promoter- associated E2F4-RBL2-HDAC1- BRM complex contributes to repression of pluripotency stem cell factors in human monocytes
Ewelina Wisnik, Tomasz Ploszaj & Agnieszka Robaszkiewicz|Department of Biophysics of Environmental Pollution, Institute of Biophysics, University of Lodz, Pomorska 141/143, 90-236, Lodz, Poland, Department of Molecular Biology, Medical university of Lodz, Narutowicza 60, 90-236, Lodz, Poland, Department of General Biophysics, Institute of biophysics, University of Lodz, Pomorska 141/143, 90-236, Lodz, Poland|2017|Scientific Reports|7:9483 DOI:10.1038/s41598-017-10307-z
Poly (ADP-Ribose) Polymerase 1 (PARP1) is a highly conserved, multifunctional enzyme found in eukaryotes. There is currently very limited knowledge about regulation of PARP1 transcription. In this study, transcriptional analysis using relative quantification was performed using the Mic qPCR Machine and its complementary micPCR software. The relative quantification was based on REST, taking target efficiency into account. The expression of a target gene is standardised by a non-regulated reference-gene (ACTB), therefore containing more reference-genes than other relative quantification methods. The expression of PARP1, POU5F1, NANOG, SOX2 and ZFP42 between differentiated monocytes and their proliferating precursors was compared. It was observed that PARP1 transcription is tightly controlled by the cell cycle and was substantially lower in monocytes. It was also found that PARP1 transcription is downregulated by RB-E2F-HDAC1-SWI/SNF-based complexes in a growth inhibition-dependent manner. The study concluded that downregulation of PARP1 transcription was observed and shown to facilitate repression of pluripotent transcription factors in human monocytes.