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Australia: A propidium monoazide–qPCR (PMA-qPCR) method for detection of viable Ascaris ova in wastewater

A propidium monoazide–qPCR (PMA-qPCR) method was validated using the Mic qPCR cycler for detecting viable Ascaris ova in wastewater

Poland: qPCR for the detection of Legionella spp. and E. Coli in hot tap water systems

Poland: qPCR for the detection of Legionella spp. and E. Coli in hot tap water systems

In this study, L. spp., L. pneumophila, and E. coli bacteria were quantified from total DNA extracts of hot tap water samples using the MIC qPCR platform with TaqMan probes. The obtained results prove the presence of these bacteria in hot tap water installations in the majority of the examined buildings.

Finland: Molecular point-of-care testing for rapid diagnosis of common colds occurring in elite athletes

Finland: Molecular point-of-care testing for rapid diagnosis of common colds occurring in elite athletes

Molecular point-of-care testing (POCT) consists of a multiplex qPCR for rapid diagnosis of the aetiological agent causing the common cold. It allows for effective treatment and enhance timely isolation to decrease viral transmission.

Iran & USA: GO/Au nanocomposite enhances amplification in PCR systems

Iran & USA: GO/Au nanocomposite enhances amplification in PCR systems

The effect of GO/Au nanocomposite was studied on end-point and real-time PCR (performed on the Mic Real-Time Cycler) employed for amplification of human GAPDH gene. The results showed that the GO/Au nanocomposite can improve both end-point and real time PCR methods at optimum concentrations, possibly through providing increased thermal convection by the GO surface as well as the Au nanostructures.

USA & China: Noninflammatory changes in Microglia contribute to epileptogenesis

USA & China: Noninflammatory changes in Microglia contribute to epileptogenesis

The effects of electroconvulsive shock on microglia was measured by mRNA expression analysis, using two-step RT-qPCR performed on the Mic Real-Time Cycler. The results showed physiological changes in microglia but no neural injury, which may be relevant to electroconvulsive therapy.

Hungary: Severe kidney disease in Malaysian broiler chickens

Hungary: Severe kidney disease in Malaysian broiler chickens

A RT-qPCR, performed on the Mic Real-Time Cycler, was used to validate the identification of the etiological agent causing severe kidney disease in broiler chickens. The virus detected was found to be the virus Kedah fatal kidney syndrome virus (KFKSV).

USA & China: Rapid diagnostic kit for accurate assessment of antifungal resistance of C. auris

USA & China: Rapid diagnostic kit for accurate assessment of antifungal resistance of C. auris

A rapid diagnostic kit was developed for accurate and rapid assessment of FKS1-associated echinocandin resistance and ERG11-associated azole resistance in C. auris. The diagnostic platform used a duplex asymmetric PCR in conjunction with molecular beacon probe-based melting curve analysis that was performed on the Mic Real-Time PCR cycler.

Switzerland: ChIP assay for study of specific-nuclear signaling factors

Switzerland: ChIP assay for study of specific-nuclear signaling factors

In this study, a TF-ChIP protocol was developed to study specific nuclear signalling factors in cancers. The protocol produces high quality DNA output and can be reproduced using both fresh and fresh-frozen tissues, which is important in a clinical setting.

Canada & USA: Pathogenesis of Phytophthora sojae on soybean

Canada & USA: Pathogenesis of Phytophthora sojae on soybean

Gene expression of the pathogenic genes in Phytophthora sojae were studied to further understand the gene-for-gene interaction between the pathogen and resistant soybean genes. In this study, a RT-qPCR was performed on the MIC and found that genomic signatures can predict phenotype compatibility between these genes.

Poland: Plant miRNAs in human breast milk

Poland: Plant miRNAs in human breast milk

RT-qPCR experiments were performed on the MIC to identify the presence of five plant food-derived miRNAs that are present in human breast milk. Two of these miRNAs were found to be in exosomes and may influence crucial biological pathways in infants.