TF-ChIP Method for Tissue-Specific Gene Targets
Amalia Perna and Lavinia Auber Alberi | Department of Medicine, University of Fribourg, Fribourg, Switzerland, Swiss Integrative Center for Human Health, Fribourg, Switzerland | 2019 | Frontiers in Cellular Neuroscience;13:95 doi: 10.3389/fncel.2019.00095
Chromatin immunoprecipitation (ChIP) assays allows transcription processes to be analysed. As transcription processes are important in adaptive and maladaptive responses in cancers and neurodegeneration, it is important to use this technique to study specific nuclear signalling factors (TFs) in cancers. The protocol published in this study produces high quality DNA for further sequencing or hybridisation and can be reproduced using both fresh and fresh-frozen tissue, which is important for clinical settings. Subsequently to the ChIP procedure, qPCR analysis is undertaken to investigate the protein-DNA interaction at genomic binding sites to provide an accurate determination of levels of specific DNA in ChIP-ed samples as well as confirmation of specificity.
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