A New Antibacterial Agent-Releasing Polydimethylsiloxane Coating for Polymethyl Methacrylate Dental Restorations
Hang-Nga Mai, Do-Yeon Kim, Dong Choon Hyun, Ju Hayng Park, Sang Min Lee and Du-Hyeong Lee|Department of Prosthodontics, School of Dentistry, Institute for Translational Research in Dentisty, Kyungpook National University, Daegu 41940, Korea; Department of Pharmacology, School of Dentistry, Kyungpook National University, Daegu 41940, Korea; Department of Polymer Science and Engineering, Kyungpook National University, Daegu 41940, Korea|Journal of Clinical Medicine|2019|8, 1831; doi:10.3390/jcm8111831
Polymethyl methacrylate (PMMA) is a biocompatible and nondegradable acrylic resin that is frequently used in restorative material in the dental industry. PMMA restorations tend to have more surface defects, higher porosity and water absorptiveness compared to metal or ceramic restorations. This can lead to the development of micropores which can act as potential incubation chambers for bacteria, resulting in inflammation in the gingival tissue. In this study, a novel chlorhexidine (CHX)-releasing polydimethylsiloxane (PDMS) coating for PMMA restorations was developed. Mesoporous silica nanoparticles (MSNs) were used to encapsulate the CHX before being delivered to PDMS. MSNs provide superior physiochemical properties, excellent biocompatibility, cost effectiveness and high loading capacity for various biomolecules due to their high porosity. The loading and release capacity of CHX was measured using liquid chromatography and the biological safety was evaluated using cytotoxicity tests. Relative quantification was performed on the Mic qPCR Cycler to analyse the intracellular molecular events. The specimens did not alter the phosphor-ERK signalling activation or phosphor-Histone 3 (ser10) levels, confirming that the specimens did not affect the cellular mitotic activity in general. To conclude, the CHX-loaded PDMS-based antibacterial coating for PMMA restorations shows a slow and stable release of CHX. The cell viability assay showed the non-toxicity of the synthesized coating substance.